KNAW Repository

Comparative-Analysis of Gene-Sequences Encoding Ammonia Monooxygenase of Nitrosospira Sp Ahb1 and Nitrosolobus- Multiformis C-71

Rotthauwe, J.H. and De Boer, W. and Liesack, W. (1995) Comparative-Analysis of Gene-Sequences Encoding Ammonia Monooxygenase of Nitrosospira Sp Ahb1 and Nitrosolobus- Multiformis C-71. FEMS Microbiology Letters, 133, 131-135. ISSN 0378-1097.

[img]PDF - Published Version
Restricted to KNAW only

271Kb

Official URL: http://dx.doi.org/10.1016/0378-1097(95)00349-A

Abstract

DNA encoding ammonia monooxygenase from two phylogenetically related autotrophic nitrifying bacteria, Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71, was amplified by PCR, The resulting products were cloned into the vector pCR-Script, A continuous region of DNA of about 1.5 kb for strain AHB1 and 1.24 kb for N. multiformis C-71 was analysed. These comprised the major part of the gene amoA encoding the active site polypeptide and, directly downstream, the 5' portion of the amoB gene. The identity values for these sequences at the amino acid level were 93.0% for amoA and 96.1% for amoB. The corresponding values for the nucleic acid sequences were 86.7% and 88.8%, respectively. The identity of the 16S rRNA gene of strain AHB1 to that of N. multiformis C-71 was at least 98.5%. The different degree of sequence conservation between the 16S rDNA and the genes encoding for ammonia monooxygenase facilitates the application of the latter as a molecular tool for a fine-scale differentiation of autotrophic nitrifying bacteria, at the species or strain level, in both environmental and cultivation studies. [KEYWORDS: Nitrosospira sp ahb1; nitrosolobus multiformis c-71; ammonia monooxygenase genes; 16s rdna Nitrification]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:10347
Deposited On:25 Nov 2011 01:00
Last Modified:31 Mar 2014 10:10

Repository Staff Only: item control page