De Graaf, W. and Cappenberg, T.E. (1996) Evidence for isotopic exchange during metabolism of stable- isotope-labeled formate in a methanogenic sediment. Applied and Environmental Microbiology, 62, 3535-3537. ISSN 0099-2240.
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Official URL: http://aem.asm.org/cgi/content/abstract/62/9/3535
The disappearance of stable-isotope-labeled formate in freshwater sediment from Lake Vechten was studied by gas chromatography-mass spectrometry. Both deuterium- and C-13- labeled formate mere removed from pore water at a high rate (400 +/- 35 [standard deviation] and 1,700 +/- 600 mu M h(-1), respectively), with concomitant formation of nonlabeled formate. The removal rate of labeled and nonlabeled formate amounted to 240 +/- 15 and 400 +/- 40 mu M h(-1), respectively. The formation of nonlabeled formate is explained by isotopic exchange due to the activity of formate dehydrogenase rather than by the turnover of formate. [KEYWORDS: Sulfate-reducing bacteria; marine sediment; acetate; turnover; hydrogen; lake]
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