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UVC-induced lysis and detritus production of Oscillatoria limnetica in a two-stage continuous-flow system

Van Hannen, E.J. and Gons, H.J. (1997) UVC-induced lysis and detritus production of Oscillatoria limnetica in a two-stage continuous-flow system. Journal of Plankton Research, 19, 723-733. ISSN 0142-7873.

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Official URL: http://dx.doi.org/10.1093/plankt/19.6.723

Abstract

In order to study in model systems the role of heterotrophic micro-organisms in the aquatic microbial food web, a natural food source consisting of senescent primary producer cells is indispensable. A two-stage continuous-flow system with the ability to produce detritus continuously is presented. In the first stage, a primary producer, i.e. the cyanobacterium Oscillatoria limnetica, was cultured. The second stage was fed with UVC-killed primary producer cells from the first stage. A control second stage received living primary producer cells. Both second stages were filled with prefiltered lake water in order to provide a natural inoculum of bacteria. During a 2 month experiment, the system was tested for its ability to produce detritus (first stage of detritus, i.e. lysis of the cell material). Chlorophyll a concentrations proved to be adequate for calculating specific lysis rates. Very high specific lysis rates were found after killing the cells with UVC as compared to the control stage. [KEYWORDS: Phytoplankton detritus; lake phytoplankton; oligotrophic lake; fresh-water; decomposition; bacteria; dynamics; algae]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:10561
Deposited On:25 Nov 2011 01:00
Last Modified:31 Mar 2014 11:01

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