Wolters J., D. and Van Dijk, C. and Zoetendal G., E. and Akkermans D. L., A. (1997) Phylogenetic characterization of ineffective Frankia in Alnus glutinosa (L.) Gaertn. nodules from wetland soil inoculants. Molecular Ecology, 6, 971-981. ISSN 0962-1083.
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Official URL: http://dx.doi.org/10.1046/j.1365-294X.1997.00265.x
Ineffective Frankia endophytes were retrieved from various wet soils by using Alnus glutinosa clones as trapping plants. No pure cultures could be isolated from these ineffective nodules. Therefore, the phylogenetic position of these endophytes was determined by sequence analysis of cloned PCR products of bacterial 16S rDNA, derived from nodules. The results showed that all nodule endophytes belong to a hitherto undescribed cluster of the Fuankia phylogenetic tree. The position of these uncultured ineffective Frankia nodule endophytes is different from that of the ineffective Fuankia isolates derived from A. glutinosa nodules, even when originating from the same geographical location. This suggests a bias in current isolation techniques. [KEYWORDS: ineffective Frankia; Alnus; 16S rDNA; phylogeny 16s ribosomal-rna; oligonucleotide probes; datisca-cannabina; genetic diversity; genus frankia; strain type; database]
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