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Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers

Boschker, H.T.S. and Nold, s.c. and Wellsbury, P. and Bos, D. and De Graaf, W. and Pel, R. and Parkes, R.J. and Cappenberg, T.E. (1998) Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers. Nature, 392, 801-805. ISSN 0028-0836.

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Recent advances in the application of molecular genetic approaches have emphasized our potentially huge underestimate of microbial diversity in a range of natural environments(1). These approaches, however, give no direct information about the biogeochemical processes in which microorganisms are active(2). Here we describe an approach to directly link specific environmental microbial processes with the organisms involved, based on the stable-carbon-isotope labelling of individual lipid biomarkers. We demonstrate this approach in aquatic sediments and provide evidence for the identity of the bacteria involved in two important biogeochemical processes: sulphate reduction coupled to acetate oxidation in estuarine and brackish sediments(3,4), and methane oxidation in a freshwater sediment(5). Our results suggest that acetate added in a C-13- labelled form was predominantly consumed by sulphate-reducing bacteria similar to the Gram-positive Desulfotomaculum acetoxidans and not by a population of the more widely studied Gram-negative Desulfobacter spp. Furthermore, C-13-methane labelling experiments suggest that type I methanotrophic bacteria dominate methane oxidation at the freshwater site. [KEYWORDS: Sulfate-reducing bacteria; gradient gel-electrophoresis; fatty-acid profiles; ribosomal-rna; methanotrophic bacteria; estuarine sediments; marine-sediments; sp-nov; acetate; desulfovibrio]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:10601
Deposited On:25 Nov 2011 01:00
Last Modified:15 Jan 2014 09:34

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