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Laser photoacoustic trace gas detection, an extremely sensitive technique applied in biological research

te Lintel Hekkert, S. and Staal, M.J. and Nabben, R.H.M. and Zuckermann, H. and Persijn, S. and Stal, L.J. and Voesenek, L.A.C.J. and Harren, F.J.M. and Reuss, J. and Parker, D.H. (1998) Laser photoacoustic trace gas detection, an extremely sensitive technique applied in biological research. Instrumentation Science and Technology, 26, 157-175. ISSN 1073-9149.

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Official URL: http://dx.doi.org/10.1080/10739149808002690

Abstract

The use of infrared laser based photo-acoustic trace gas detection equipment in biological research is discussed on the basis bf two examples. A CO2 laser based photo-acoustic trace gas detection system is employed to follow the time-dependent pattern of the nitrogen fixation process by the cyanobacteria Nodularia Spumigena on a one-minute time scale. Due to the high sensitivity of the detection system for ethylene (detection limit 6 part per trillion; 6.10(12)), the fixation process can be followed on-line in a flow-through system. Following a 50 h dark incubation period, the bacteria show nitrogen fixation only after a certain illumination period, indicating lack of carbohydrates needed to start the nitrogen fixation. [KEYWORDS: CO-LASER; PLANTS; ACETALDEHYDE; SPECTROSCOPY; ADAPTATIONS; CO2-LASER; RESPONSES; ETHYLENE; ECOLOGY; ANOXIA]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:10681
Deposited On:25 Nov 2011 01:00
Last Modified:31 Mar 2014 10:59

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