KNAW Repository

Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment

Nold, s.c. and Boschker, H.T.S. and Pel, R. and Laanbroek, H.J. (1999) Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment. FEMS Microbiology Ecology, 29, 81-89. ISSN 0168-6496.

[img]PDF - Published Version
Restricted to KNAW only

196Kb

Official URL: http://dx.doi.org/10.1016/S0168-6496(99)00002-1

Abstract

To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with (CH4)-C-13 and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assessed by quantifying the isotopic composition of the carbon in membrane lipids. The 16-carbon rather than the 18-carbon methanotroph-specific biomarkers showed a clear enrichment in C-13, suggesting the importance of group I methanotrophs in these sediments. Ammonium addition resulted in a depleted isotopic signal compared to ambient controls, suggesting that high ammonium concentrations inhibit methane incorporation into cellular components. These results compare favorably with studies that showed ammonium inhibition of methane oxidation, and extend these findings by demonstrating the effect of nitrogen fertilization on methanotroph lipid synthesis. [KEYWORDS: methane; freshwater; sediment; ammonium; lipid; stable carbon isotope Methane oxidation; fatty-acid; atmospheric methane; community structure; loosdrecht lakes; peaty sediments; eutrophic lake; bacteria; carbon; nitrification]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:10768
Deposited On:25 Nov 2011 01:00
Last Modified:31 Mar 2014 10:02

Repository Staff Only: item control page