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The blue-colored linker polypeptide L-55 is a fusion protein of phycobiliproteins in the cyanobacterium Synechocystis sp strain BO 8402

Neuschaefer-Rube, O. and Westermann, M. and Bluggel, M. and Meyer, H.E. and Ernst, A. (2000) The blue-colored linker polypeptide L-55 is a fusion protein of phycobiliproteins in the cyanobacterium Synechocystis sp strain BO 8402. European Journal of Biochemistry, 267, 3623-3632. ISSN 0014-2956.

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Official URL: http://dx.doi.org/10.1046/j.1432-1327.2000.01391.x

Abstract

The cyanobacterium Synechocystis sp. strain BO 8402, isolated from Lake Constance, lacks phycobilisomes but instead forms inclusion bodies containing remnants of phycobiliproteins. The inclusion bodies are surrounded by a proteinaceous capsule and contain alpha-phycocyanin and beta-phycocyanin, the rod linker polypeptide (LRPC)-P-35 and a novel blue-colored protein L-55 with an apparent molecular mass of 55 kDa. An antibody raised against beta-phycocyanin showed a strong cross-reaction with L- 55. Mass spectrometry analysis of proteolytic peptides from L- 55 revealed mass identity to proteolytic peptides derived from (LRPC)-P-35 and beta-phycocyanin. However, analysis of the genome of strain BO 8402 revealed only one cpcBACE operon, encoding the apoproteins of beta-phycocyanin and alpha- phycocyanin, (LRPC)-P-35 and a subunit of the phycocyanin alpha subunit phycocyanobilin lyase, respectively. The gene structure, sequence and transcription of these genes were identical to that of a revertant strain, Synechocystis sp. strain BO 9201, which formed phycobilisomes and did not express L-55. Based on these observations, we concluded that L-55 did not derive from a particular gene or from a special form of mRNA-processing. We propose that L-55 is formed by post- translational fusion of (LRPC)-P-35 and beta-phycocyanin. Cross-linking may stabilize the formation of the large paracrystalline phycocyanin aggregates unique to Synechocystis sp. strain BO 8402. [KEYWORDS: cross-link; inclusion body; phycobiliproteins;post- translational fusion; Synechocystis sp strain; BO 8402 Mastigocladus-laminosus phycobilisomes; subunit phycocyanobilinlyase; light-harvesting complexes; crystal-structure analysis;c-phycocyanin; rod substructures; core substructure; bilin attachment; gamma-subunit; 2.2 angstrom]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:10903
Deposited On:24 Nov 2011 01:00
Last Modified:31 Mar 2014 10:25

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