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Influence of fungal-bacterial interactions on bacterial conjugation in the residuesphere

Sengelov, G. and Kowalchuk, G.A. and Sørensen, S.J. (2000) Influence of fungal-bacterial interactions on bacterial conjugation in the residuesphere. FEMS Microbiology Ecology, 31, 39-45. ISSN 0168-6496.

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Conjugal gene transfer among bacteria in the residuesphere (area between decaying plant material and soil) of leaves of barley straw was studied. The residuesphere was shown to be a hot-spot for conjugal gene transfer compared to conjugation in sterile sand and non-sterile bulk soil. Impact of fungal colonisation of the residuesphere on bacterial colonisation and conjugation was also investigated. The inhibition of fungal colonisation, due to the application of an eukaryotic inhibitor, increased bacterial colonisation of the residuesphere in soil microcosms compared to non-treated leaves. This treatment also had a transient, positive effect on conjugation. Bacterial conjugation in the residuesphere of leaves subjected to 17 days of fungal colonisation was significantly lower than in the residuesphere of non-colonised leaves. Fungal biomass, as measured by chitinase activity, was inversely related to the conjugation efficiency. [KEYWORDS: soil microcosm; gene transfer; microbial colonisation; litter; Pseudomonas putida Gene-transfer; pseudomonas-putida; tol plasmid; soil; rhizosphere]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:10919
Deposited On:24 Nov 2011 01:00
Last Modified:15 Jan 2014 09:33

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