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Nitrification in the Schelde estuary: methodological aspects and factors influencing its activity

De Bie, M.J.M. and Starink, M. and Boschker, H.T.S. and Peene, J. and Laanbroek, H.J. (2002) Nitrification in the Schelde estuary: methodological aspects and factors influencing its activity. FEMS Microbiology Ecology, 42, 99-107. ISSN 0168-6496.

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Official URL: http://dx.doi.org/10.1016/S0168-6496(02)00325-2

Abstract

We present a 15-month dataset on nitrification measurements in the Schelde estuary (Belgium and The Netherlands). Nitrification was estimated using the N-serve sensitive dark 14C-bicarbonate incorporation technique. A peak of nitrification activity was observed in the freshwater part of the estuary. Downstream from this peak, nitrification declined, probably because of ammonium limitation. A range of nitrification inhibitors was tested on both a Nitrosomonas europaea culture and estuarine samples. It was found that methyl fluoride and acetylene stimulated dark 14C-bicarbonate incorporation and those inhibitors were therefore considered inappropriate nitrification inhibitors in combination with this technique. The effect of the inhibitor N-serve was studied on the dark incorporation of 13C-bicarbonate into polar lipid derived fatty acids to further identify the dominant chemoautotrophic processes. Inhibition of polar lipid derived fatty acid labelling in the presence of N-serve was complete, suggesting that nitrifying bacteria dominated the chemoautotrophic community. [KEYWORDS: Nitrification; Estuary; Polar lipid derived fatty acid; Nitrification inhibitor; Ammonium concentration]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:11133
Deposited On:24 Nov 2011 01:00
Last Modified:31 Mar 2014 10:09

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