KNAW Repository

Interference of an apcA insertion with complementary chromatic adaptation in the diazotrophic Synechocystis sp. strain BO 8402

Neuschaefer-Rube, O. and Böger, P. and Ernst, A. (2002) Interference of an apcA insertion with complementary chromatic adaptation in the diazotrophic Synechocystis sp. strain BO 8402. Biochimica et Biophysica Acta, 1553, 279-295. ISSN 0167-4889.

[img]PDF - Published Version
Restricted to KNAW only

740Kb

Official URL: http://dx.doi.org/10.1016/S0005-2728(01)00249-3

Abstract

Complementary chromatic adaptation was studied in two unicellular diazotrophic Synechocystis-type cyanobacteria. strains BO 8402 and BO 9201. Strain BO 8402 was isolated from Lake Constance as a mutant lacking phycobilisomes due to an insertion sequence element in the gene apcA. encoding alpha-allophycocyanin. Strain BO 9201 recovered the ability to assemble functional phycobilisomes after a spontaneous excision of the insertion sequence element in apcA. Simultaneously, the strain became able to perform group 11 complementary chromatic adaptation by regulating the synthesis of phycoerythrin. The two strains had identical phycoerythrin operons, cpeBA. and similar-sized transcripts were formed upon induction by green light, However. in strain BO 8402 the cpeBA transcript level was approx. 20-fold lower than in strain BO 9201. Because strain BO 8402 cannot synthesize allophycocyanin and phycocyanin is sequestered in paracrystalline inclusion bodies, non-assembled phycoerythrin may accumulate inside the cells. It as examined whether non-assembled phycoerythrin or other effects caused by the absence of phycobilisomes. such as a permanently oxidized redox status of the photosynthetic electron transport chain or a distorted ratio of C and N assimilation mediated the repression of cpeBA transcription in strain BO 8402. No such links could be established. We therefore concluded that in these diazotrophic Synechocystis-type cyanobacteria the green light-induced transcription of the cpe operon directly required a functional apc operon [KEYWORDS: nitrogen metabolism, phycoerythrin, complementary chromatic adaptation, internal signaling, cyanobacterium, Synechocystis]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:11196
Deposited On:24 Nov 2011 01:00
Last Modified:31 Mar 2014 10:04

Repository Staff Only: item control page