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Development and characterisation of microsatellite markers for Liporrhopalum tentacularis Grandi, the pollinator fig wasp of Ficus montana Blume

Zavodna, M. and Arens, P. and Vosman, B. and Van Dijk, P.J. and Van Damme, J.M.M. (2002) Development and characterisation of microsatellite markers for Liporrhopalum tentacularis Grandi, the pollinator fig wasp of Ficus montana Blume. Molecular Ecology Notes, 2, 569-571. ISSN 1471-8278.

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Official URL: http://dx.doi.org/10.1046/j.1471-8286.2002.00319.x

Abstract

Microsatellite markers for the pollinator fig wasp Liporrhopalum tentacularis were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. A subset of 31 positive clones was sequenced and primers were designed. Eleven primer pairs produced polymorphic amplification products in L. tentacularis. Eight markers gave unambiguously scorable patterns and were further characterized on 29 individuals collected from different fruits of the dioecious host fig Ficus montana in Indonesia. Three to 19 alleles per locus were detected in this set of samples. The observed heterozygosity ranged between 0.10 and 0.55.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:11264
Deposited On:24 Nov 2011 01:00
Last Modified:31 Mar 2014 10:55

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