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Competition of a parathion-hydrolyzing Flavobacterium with bacteria from ditch water in carbon-, nitrate- and phosphate-limited continuous cultures

Sprenger, W. and Dijkstra, A. and Zwart, G. and Van Agterveld, M.P. and Van Noort, P.C.M. and Parsons, J.R. (2003) Competition of a parathion-hydrolyzing Flavobacterium with bacteria from ditch water in carbon-, nitrate- and phosphate-limited continuous cultures. FEMS Microbiology Ecology, 43, 45-53. ISSN 0168-6496.

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Official URL: http://dx.doi.org/10.1016/S0168-6496(02)00415-4

Abstract

The effect of competition for macroelements with bacteria from ditch water on the parathion-hydrolyzing Flavobacterium sp. ATCC 27551 (FB) was investigated within mixed continuous cultures under carbon-, nitrate- or phosphate-limited conditions. The high initial rate of parathion hydrolysis decreased rapidly in all cultures due to the loss of strain FB. Addition of 2-isopropyl-6-methyl-4-pyrimidinol (a selective source of carbon, nitrogen and energy for FB) to one nitrate- and carbon-limited chemostat caused a 20-fold increase in parathion-hydrolyzing activity compared to unamended control cultures and retention of FB. The presence of the parathion hydrolase-encoding gene could be demonstrated by a newly developed PCR detection method in all FB cultures during most of the cultivation period. These results suggest that competition effects cause the pesticide-degrading capacity of microbial communities depending on their frequency of exposure to the pesticide compounds. [KEYWORDS: Organophosphorus; Pesticide; Biodegradation; Nutrient; Competition; Chemostat]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:11401
Deposited On:24 Nov 2011 01:00
Last Modified:31 Mar 2014 10:29

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