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Development of extraradical mycelium of Scutellospora reticulata under root-organ culture : spore production and function of auxiliary cells

Declerck, S. and D'Or, D. and Bivort, C. and Souza. de, F. A. (2004) Development of extraradical mycelium of Scutellospora reticulata under root-organ culture : spore production and function of auxiliary cells. Mycological Research, 108, 84-92. ISSN 0953-7562.

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Official URL: http://dx.doi.org/10.1017/S0953756203008761

Abstract

The development of the extraradical mycelium and auxiliary cells and spore production of Scutellospora reticulata in association with Ri T-DNA transformed carrot roots was followed under root-organ culture conditions. Extraradical mycelium development followed classical lag-exponential-plateau phases, with an additional late decline phase in number of auxiliary cells. Spore production started in parallel with a critical extraradical mycelium biomass produced, continued long after root growth ceased and during the late decline in auxiliary cells number. Isolated auxiliary cells were shown to exhibit hyphal re-growth, but not root colonization, either in situ or in vitro. These results showed that root and extraradical mycelium development were intimately associated in a sequence where both grew together during active root growth, followed during root aging by a period in which only the fungus developed. Spore production appeared dependent on a critical extraradical mycelium biomass and on the re-allocation of resources from both the intraradical mycelium and the auxiliary cells via the hyphal network.

Item Type:Article
ID Code:11503
Deposited On:23 Nov 2011 01:00
Last Modified:24 Apr 2012 16:33

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