KNAW Repository

A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis

Janse, I. and Bok, J.M. and Zwart, G. (2004) A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis. Journal of Microbiological Methods, 57, 279-281. ISSN 0167-7012.

[img]PDF - Published Version
Restricted to KNAW only

108Kb

Official URL: http://dx.doi.org/10.1016/j.mimet.2003.12.006

Abstract

Denaturant gradient gel electrophoresis (DGGE) is a widely used method for mutation analysis and for studies of microbial diversity. Particular combinations of target gene fragments and primers may give rise to erroneous DGGE profiles. We report on a very straightforward means to eliminate the artifactual ‘double bands’ that can be encountered in several applications. [KEYWORDS: DGGE ; Double bands ; Artifacts]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:11534
Deposited On:23 Nov 2011 01:00
Last Modified:31 Mar 2014 10:30

Repository Staff Only: item control page