Janse, I. and Bok, J.M. and Zwart, G. (2004) A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis. Journal of Microbiological Methods, 279-281. ISSN 0167-7012.
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Official URL: http://dx.doi.org/10.1016/j.mimet.2003.12.006
Abstract
Denaturant gradient gel electrophoresis (DGGE) is a widely used method for mutation analysis and for studies of microbial diversity. Particular combinations of target gene fragments and primers may give rise to erroneous DGGE profiles. We report on a very straightforward means to eliminate the artifactual ‘double bands’ that can be encountered in several applications. [KEYWORDS: DGGE ; Double bands ; Artifacts]
| Item Type: | Article |
|---|---|
| Institutes: | Nederlands Instituut voor Ecologie (NIOO) |
| ID Code: | 11534 |
| Deposited On: | 23 Nov 2011 01:00 |
| Last Modified: | 24 Apr 2012 16:33 |
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