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Morphological, ontogenetic and molecular characterization of Scutellospora reticulata (Glomeromycota)

De Souza, F.A. and Declerck, S. and Smit, E. and Kowalchuk, G.A. (2005) Morphological, ontogenetic and molecular characterization of Scutellospora reticulata (Glomeromycota). Mycological Research, 109, 697-706. ISSN 0953-7562.

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Official URL: http://dx.doi.org/10.1017/S0953756205002546

Abstract

The arbuscular mycorrhizal (AM) fungus Scutellospora reticulata (CNPAB11) was characterized using morphological, ontogenetic and molecular approaches. Spore ontogenesis was studied using Ri T-DNA transformed carrot roots and observations were compared with those published for eight other, pot-cultured, Scutellospora species. The sporogenesis of S. reticulata exhibited an unreported pattern of outer spore wall differentiation. In addition, Denaturing Gradient Gel Electrophoresis (DGGE), targeting the V9 region of the SSU nrDNA, was used to differentiate S. reticulata from 16 other Scutellospora species and results were confirmed by sequencing analysis. Phylogenetic analyses, using nearly full length SSU nrDNA sequences, grouped S. reticulata in a cluster together with S. cerradensis and S. heterogama, species that share similar spore wall organization and also possess ornamented external walls. PCR-DGGE and sequence analysis revealed intragenomic SSU nrDNA polymorphisms in four out of six Scutellospora species tested, and demonstrated that SSU nrDNA intragenomic polymorphism could be used as a marker to differentiate several closely related Scutellospora species.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:11686
Deposited On:23 Nov 2011 01:00
Last Modified:31 Mar 2014 10:09

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