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Development and characterization of microsatellite markers for two dioecious Ficus species

Zavodna, M. and Arens, P. and Dijk van, P.J. and Vosman, B. (2005) Development and characterization of microsatellite markers for two dioecious Ficus species. Molecular Ecology Notes, 5, 355-357. ISSN 1471-8278.

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Official URL: http://dx.doi.org/10.1111/j.1471-8286.2005.00924.x

Abstract

Microsatellite markers for Ficus montana and Ficus septica were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. The subsets of five and three best scorable primer pairs were characterized on 24 F. montana and 36 F. septica individuals, respectively. For F. montana, loci showed five to 14 alleles per locus and expected heterozygosities ranged between 0.23 and 0.87. For F. septica, loci showed three to five alleles per locus and expected heterozygosities ranged between 0.36 and 0.49. Four primer pairs (two from each subset) cross-amplified in the other species, indicating transportability of the markers within the genus Ficus. [KEYWORDS: dispersal ; Ficus montana ; Ficus septica ; mutualism ; pollinating fig wasps ; simple sequence repeat (SSR)]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:11829
Deposited On:23 Nov 2011 01:00
Last Modified:31 Mar 2014 09:58

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