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Functional recovery of biofilm bacterial communities after copper exposure

Boivin, M.E.Y. and Massieux, B. and Breure, A.M. and Greve, G.D. and Rutgers, M. and Admiraal, W. (2006) Functional recovery of biofilm bacterial communities after copper exposure. Environmental Pollution, 140, 239-246. ISSN 0269-7491.

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Official URL: http://dx.doi.org/10.1016/j.envpol.2005.07.014

Abstract

Potential of bacterial Communities in biofilms to recover after copper exposure was investigated. Biofilms grown outdoor in shallow water on glass dishes were exposed ill the laboratory to 0.6, 2.1, 6.8 mu mol/l copper amended surface water and a reference and subsequently to un-amended surface water. Transitions of bacterial communities were characterised with denaturing gradient gel electrophoresis (DGGE) and community-level physiological profiles (CLPP). Exposure to 6.8 mu mol/l copper provoked distinct changes in DGGE profiles of bacterial consortia, which did not reverse upon copper depuration. Exposure to 2.1 and 6.8 mu mol/l copper was found to induce marked changes ill CLPP of bacterial communities that proved to be reversible during copper depuration. Furthermore, copper exposure induced the development of copper-tolerance, which was partially lost during depuration. It is concluded that bacterial communities exposed to copper contaminated water for a period of 26 days are capable to restore their metabolic attributes after introduction of unpolluted water in aquaria for 28 days. [KEYWORDS: pollution-induced community tolerance (PICT) ; community-level physiological profiling (CLPP) ; denaturing gradient gel electrophoresis (DGGE) ; bacterial communities ; recovery]

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:11908
Deposited On:16 Nov 2011 01:00
Last Modified:31 Mar 2014 10:40

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