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Chromothripsis is a common mechanism driving genomic rearrangements in primary and metastatic colorectal cancer

Kloosterman, W.P. and Hoogstraat, M. and Paling, O. and Tavakoli-Yaraki, M. and Renkens, I. and Vermaat, J.E. and Roosmalen van, M. and Lieshout van, S. and Nijman, I.J. and Roessingh, W. and Slot Van't, R. and Belt van de, J. and Guryev, V. and Koudijs, M.J. and Voest, E.E. and Cuppen, E. (2011) Chromothripsis is a common mechanism driving genomic rearrangements in primary and metastatic colorectal cancer. Genome Biology, 12, R103-. ISSN 1474-760X.

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ABSTRACT: BACKGROUND: Structural rearrangements form a major class of somatic variation in cancer genomes. Local chromosome shattering, termed chromothripsis, is a mechanism proposed to be the cause of clustered chromosomal rearrangements and was recently described to occur in a small percentage of tumors. The significance of these clusters for tumor development or metastatic spread is largely unclear. RESULTS: We used genome-wide long mate-pair sequencing and SNP array profiling to reveal that chromothripsis is a widespread phenomenon in primary colorectal cancer and metastases. We find large and small chromothripsis events in nearly every colorectal tumor sample and show that several breakpoints of chromothripsis clusters and isolated rearrangements affect cancer genes, including NOTCH2, EXO1 and MLL3. We complemented the structural variation studies by sequencing the coding regions of a cancer exome in all colorectal tumor samples and found somatic mutations in 24 genes, including APC, KRAS, SMAD4 and PIK3CA. A pairwise comparison of somatic variations in primary and metastatic samples indicated that many chromothripsis clusters, isolated rearrangements and point mutations are exclusively present in either the primary tumor or the metastasis and may affect cancer genes in a lesion-specific manner. CONCLUSIONS: We conclude that chromothripsis is a prevalent mechanism driving structural rearrangements in colorectal cancer and show that a complex interplay between point mutations, simple copy number changes and chromothripsis events drive colorectal tumor development and metastasis.

Item Type:Article
Institutes:Hubrecht Instituut
ID Code:12609
Deposited On:30 Jan 2012 01:00
Last Modified:20 Sep 2012 11:02

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