Palma Di, S. and Stange, D.E. and Wetering van de, M.L. and Clevers, H. and Heck, A. and Mohammed, S. (2011) Highly sensitive proteome analysis of FACS-sorted adult colon stem cells. Journal of Proteome Research, 10, 3814-9. ISSN 1535-3893.
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Official URL: http://dx.doi.org/10.1021/pr200367p
In proteomics, multidimensional liquid chromatography combined with mass spectrometry has become a standard technique to reduce sample complexity and tackle the vast dynamic range. Such fractionation is necessary to obtain a comprehensive analysis of biological samples such as tissues and cell lines. However, extensive fractionation comes at the expense of sample losses, hampering the analysis of limited material. We previously described a highly sensitive multidimensional chromatographic strategy based on a combination of hydrophilic interaction liquid chromatography and reversed phase chromatography, which allows proteomic analysis with minimal sample losses. Here we apply this strategy to the analysis of a limited number of FACS-sorted colon stem cells extracted from mouse intestine, obtaining a proteome coverage comparable to current methods that generally require 100-fold more starting material. We propose that this alternative multidimensional chromatographic technology will find ample application such as in the analysis of distinct cellular populations obtained by laser microdissection.
|Deposited On:||19 Sep 2012 12:46|
|Last Modified:||14 Oct 2012 19:05|
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