KNAW Repository

Evidence for post-transcriptional regulation of clustered microRNAs in Drosophila

Ryazansky, S. S. and Gvozdev, V. A. and Berezikov, E. (2011) Evidence for post-transcriptional regulation of clustered microRNAs in Drosophila. BMC Genomics, 12, 371-. ISSN 1471-2164.

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution Non-commercial Share Alike.

1665Kb

Official URL: http://dx.doi.org/10.1186/1471-2164-12-371

Abstract

BACKGROUND: MicroRNAs (miRNA) are short 21-23nt RNAs capable of inhibiting translation of complementary target messenger RNAs. Almost half of D. melanogaster miRNA genes are grouped in genomic clusters. RESULTS: The peculiarities of the expression of clustered miRNAs were studied using publicly available libraries of sequenced small RNAs from different Drosophila tissues. We have shown that although miRNAs from almost all clusters have similar tissue expression profiles (coordinated clusters), some clusters contain miRNAs with uncoordinated expression profiles. The predicted transcription start sites (TSSs) of such clusters are located upstream of the first miRNA, but no TSSs are found within the clusters. The expression profiles of miR and miR* sequences in uncoordinated clustered miRNAs do not correlate while their profiles from the coordinated clustered miRNAs are similar. CONCLUSIONS: The presence of exclusively upstream promoters in miRNA clusters containing uncoordinated miRNAs means that the clusters are transcribed as single transcription units. The difference of tissue expression profiles of uncoordinated miRNAs and the corresponding miRs* suggests a post-transcriptional regulation of their processing or stability. [KEYWORDS: Animals, Drosophila/ genetics, Gene Expression Profiling, MicroRNAs/ genetics/ metabolism, Multigene Family, Promoter Regions, Genetic, RNA Processing, Post-Transcriptional, Transcription Initiation Site]

Item Type:Article
Institutes:Hubrecht Instituut
ID Code:12649
Deposited On:19 Sep 2012 14:00
Last Modified:14 Oct 2012 19:05

Repository Staff Only: item control page