KNAW Repository

Zebrafish prox1b Mutants Develop a Lymphatic Vasculature, and prox1b Does Not Specifically Mark Lymphatic Endothelial Cells

Tao, S. and Witte de, M. and Bryson-Richardson, R.J. and Currie, P.D. and Hogan, B.M. and Schulte-Merker, S. (2011) Zebrafish prox1b Mutants Develop a Lymphatic Vasculature, and prox1b Does Not Specifically Mark Lymphatic Endothelial Cells. PLoS One, 6, e28934-. ISSN 1932-6203.

[img]
Preview
PDF - Published Version
Available under License Creative Commons Attribution Non-commercial Share Alike.

2609Kb

Official URL: http://dx.doi.org/10.1371/journal.pone.0028934

Abstract

BACKGROUND: The expression of the Prospero homeodomain transcription factor (Prox1) in a subset of cardinal venous cells specifies the lymphatic lineage in mice. Prox1 is also indispensible for the maintenance of lymphatic cell fate, and is therefore considered a master control gene for lymphangiogenesis in mammals. In zebrafish, there are two prox1 paralogues, the previously described prox1 (also known as prox1a) and the newly identified prox1b. PRINCIPAL FINDINGS: To investigate the role of the prox1b gene in zebrafish lymphangiogenesis, we knocked-down prox1b and found that depletion of prox1b mRNA did not cause lymphatic defects. We also generated two different prox1b mutant alleles, and maternal-zygotic homozygous mutant embryos were viable and did not show any lymphatic defects. Furthermore, the expression of prox1b was not restricted to lymphatic vessels during zebrafish development. CONCLUSION: We conclude that Prox1b activity is not essential for embryonic lymphatic development in zebrafish.

Item Type:Article
Institutes:Hubrecht Instituut
ID Code:12667
Deposited On:19 Sep 2012 15:38
Last Modified:14 Oct 2012 19:02

Repository Staff Only: item control page