KNAW Repository

A 3-year study reveals that plant growth stage, season and field site affect soil fungal communities while cultivar and GM-trait have minor effects

Hannula, S.E. and De Boer, W. and Van Veen, J.A. (2012) A 3-year study reveals that plant growth stage, season and field site affect soil fungal communities while cultivar and GM-trait have minor effects. PLoS One, 7, e33819-. ISSN 1932-6203.

[img]
Preview
PDF - Published Version
449Kb

Official URL: http://dx.doi.org/10.1371/journal.pone.0033819

Abstract

In this three year field study the impact of different potato (Solanum tuberosum L.) cultivars including a genetically modified (GM) amylopectin-accumulating potato line on rhizosphere fungal communities are investigated using molecular microbiological methods. The effects of growth stage of a plant, soil type and year on the rhizosphere fungi were included in this study. To compare the effects, one GM cultivar, the parental isoline, and four non-related cultivars were planted in the fields and analysed using T-RFLP on the basis of fungal phylum specific primers combined with multivariate statistical methods. Additionally, fungal biomass and some extracellular fungal enzymes (laccases, Mn-peroxidases and cellulases) were quantified in order to gain insight into the function of the fungal communities. Plant growth stage and year (and agricultural management) had the strongest effect on both diversity and function of the fungal communities while the GM-trait studied was the least explanatory factor. The impact of cultivar and soil type was intermediate. Occasional differences between cultivars, the amylopectin-accumulating potato line, and its parental variety were detected, but these differences were mostly transient in nature and detected either only in one soil, one growth stage or one year.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:12739
Deposited On:06 Nov 2012 10:50
Last Modified:22 Aug 2013 11:54

Repository Staff Only: item control page