KNAW Repository

A multi-gene phylogeny for species of Mycosphaerella occurring on Eucalyptus leaves.

Hunter, G.C. and Wingfield, B.D. and Crous, P.W. and Wingfield, M.J. (2006) A multi-gene phylogeny for species of Mycosphaerella occurring on Eucalyptus leaves. Studies in Mycology, 55, 147-161. Open Access uitgave; available: http://www.studiesinmycology.org/cgi/content/abstract/55/1/147.

[img]PDF
624Kb

Abstract

Species of the ascomycete genus Mycosphaerella are regarded as some of the most destructive leaf pathogens of a large number of economically important crop plants. Amongst these, approximately 60 Mycosphaerella spp. have been identified from various Eucalyptus spp. where they cause leaf diseases collectively known as Mycosphaerella Leaf Disease (MLD). Species concepts for this group of fungi remain confused, and hence their species identification is notoriously difficult. Thus, the introduction of DNA sequence comparisons has become the definitive characteristic used to distinguish species of Mycosphaerella. Sequences of the Internal Transcribed Spacer (ITS) region of the ribosomal RNA operon have most commonly been used to consider species boundaries in Mycosphaerella. However, sequences for this gene region do not always provide sufficient resolution for cryptic taxa. The aim of this study was, therefore, to use DNA sequences for three loci, ITS, Elongation factor 1-alpha (EF-1{alpha}) and Actin (ACT) to reconsider species boundaries for Mycosphaerella spp. from Eucalyptus. A further aim was to study the anamorph concepts and resolve the deeper nodes of Mycosphaerella, for which part of the Large Subunit (LSU) of the nuclear rRNA operon was sequenced. The ITS and EF-1{alpha} gene regions were found to be useful, but the ACT gene region did not provide species-level resolution in Mycosphaerella. A phylogeny of the combined DNA datasets showed that species of Mycosphaerella from Eucalyptus cluster in two distinct groups, which might ultimately represent discrete genera.

Item Type:Article
Additional Information:Open Access uitgave; available: http://www.studiesinmycology.org/cgi/content/abstract/55/1/147
Institutes:Centraalbureau voor Schimmelcultures (CBS)
ID Code:3658
Deposited On:13 Feb 2009 17:14
Last Modified:13 Oct 2010 22:35

Repository Staff Only: item control page