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The Intestinal Wnt/TCF Signature

Flier, L.G. van der and Sabates-Bellver, J. and Oving, I. and Haegebarth, A. and Palo, M. de and Anti, M. and Gijn, M.E. van and Suijkerbuijk, S and Wetering, M. van de and Marra, G. and Clevers, J.C. (2007) The Intestinal Wnt/TCF Signature. Gastroenterology, 132(2), 628-632. doi:10.1053/j.gastro.2006.08.039.

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BACKGROUND & AIMS: In colorectal cancer, activating mutations in the Wnt pathway transform epithelial cells through the inappropriate expression of a TCF4 target gene program, which is physiologically expressed in intestinal crypts. METHODS: We have now performed an exhaustive array-based analysis of this target gene program in colorectal cancer cell lines carrying an inducible block of the Wnt cascade. Independently, differential gene-expression profiles of human adenomas and adenocarcinomas vs normal colonic epithelium were obtained. RESULTS: Expression analyses of approximately 80 genes common between these data sets were performed in a murine adenoma model. The combined data sets describe a core target gene program, the intestinal Wnt/TCF signature gene set, which is responsible for the transformation of human intestinal epithelial cells. CONCLUSIONS: The genes were invariably expressed in adenomas, yet could be subdivided into 3 modules, based on expression in distinct crypt compartments. A module of 17 genes was specifically expressed at the position of the crypt stem cell.

Item Type:Article
Additional Information:doi:10.1053/j.gastro.2006.08.039
Institutes:Hubrecht Instituut
ID Code:3768
Deposited On:13 Feb 2009 17:14
Last Modified:10 Dec 2009 13:14

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