KNAW Repository

Planktonic primary production in estuaries: a comparison of the 14C, O2 and 18O methods

Gazeau, F.P.H. and Middelburg, J.J. and Loijens, M. and Vanderborght, J.P. and Pizay, M-D. and Gattuso, J.P. (2007) Planktonic primary production in estuaries: a comparison of the 14C, O2 and 18O methods. Aquatic Microbial Ecology, 46, 95-106. ISSN 0948-3055.

[img]PDF - Published Version
432Kb

Official URL: http://dx.doi.org/10.3354/ame046095

Abstract

Rates of primary production were measured in 2 estuaries (Randers Fjord, Denmark, and the Scheldt estuary, Belgium/The Netherlands) using 3 different incubation methods: (1) the oxygen light-dark method (O2-LD), (2) 14C incorporation and (3) 18O labeling. Estimates based on the 14C incorporation technique were not significantly different from those obtained using the O2-LD technique. The 18O approach provided rates significantly lower than the 2 other techniques. Ratios of O2-LD to 18O-based rates (range: 0.99 to 3.54) were often statistically significantly higher than 1 and increased with decreasing salinities and/or lower oxygen concentrations. The underestimation of gross primary production by the 18O method may be due to an intracellular recycling of labeled oxygen which increased in magnitude with decreasing external oxygen conditions. These results suggest that the 18O method must be used with extreme care in nutrient-rich, low oxygen systems.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:4492
Deposited On:15 Sep 2009 02:00
Last Modified:31 Mar 2014 10:50

Repository Staff Only: item control page