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Identification of genotypically diverse Cryptococcus neoformans and Cryptococcus gattii isolates by Luminex xMAP Technology

Bovers, M. and Diaz, M.R. and Hagen, F. and Spanjaard, L. and Duim, B. and Visser, C.E. and Hoogveld, H.L. and Scharringa, J. and Hoepelman, I.M. and Fell, J.W. and Boekhout, T. (2007) Identification of genotypically diverse Cryptococcus neoformans and Cryptococcus gattii isolates by Luminex xMAP Technology. Journal of Clinical Microbiology, 45, 1874-1883. ISSN 0095-1137.

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Official URL: http://dx.doi.org/10.1128/JCM.00223-07

Abstract

A Luminex suspension array, which had been developed for identification of Cryptococcus neoformans and Cryptococcus gattii isolates, was tested by genotyping a set of 58 mostly clinical isolates. All genotypes of C. neoformans and C. gattii were included. In addition, cerebrospinal fluid (CSF) obtained from patients with cryptococcal meningitis was used to investigate the feasibility of the technique for identification of the infecting strain. The suspension array correctly identified haploid isolates in all cases. Furthermore, hybrid isolates possessing two alleles of the Luminex probe region could be identified as hybrids. In CSF specimens, the genotype of the cryptococcal strains responsible for infection could be identified after optimization of the PCR conditions. However, further optimization of the DNA extraction protocol is needed to enhance the usability of the method in clinical practice.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
Centraalbureau voor Schimmelcultures (CBS)
ID Code:4603
Deposited On:16 Sep 2009 02:00
Last Modified:31 Mar 2014 10:39

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