Van Doorn, R. and Szemes, M. and Bonants, P.J.M. and Kowalchuk, G.A. and Sallas, J. and Ortenberg, E. and Schoen, C.D. (2007) Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArraysTM. BMC Genomics, 8, 276-. ISSN 1471-2164.
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Official URL: http://dx.doi.org/10.1186/1471-2164-8-276
Background Diagnostics and disease-management strategies require technologies to enable the simultaneous detection and quantification of a wide range of pathogenic microorganisms. Most multiplex, quantitative detection methods available suffer from compromises between the level of multiplexing, throughput and accuracy of quantification. Here, we demonstrate the efficacy of a novel, high-throughput, ligation-based assay for simultaneous quantitative detection of multiple plant pathogens. The ligation probes Results The performance of the PRI-lock detection system was demonstrated using 13 probes targeting several significant plant pathogens at different taxonomic levels. All probes specifically detected their corresponding targets and provided perfect discrimination against non-target organisms with very similar ligation target sites. The nucleic acid targets could be reliably quantified over 5 orders of magnitude with a dynamic detection range of more than 104. Pathogen quantification was equally robust i Conclusion This novel assay enables very specific, high-throughput, quantitative detection of multiple pathogens over a wide range of target concentrations and should be easily adaptable for versatile diagnostic purposes.
|Institutes:||Nederlands Instituut voor Ecologie (NIOO)|
|Deposited On:||16 Sep 2009 02:00|
|Last Modified:||02 Nov 2011 16:37|
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