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A versatile method for stable carbon isotope analysis of carbohydrates by high-performance liquid chromatography/isotope ratio mass spectrometry

Boschker, H.T.S. and Moerdijk-Poortvliet, T.C.W. and Van Breugel, P.A. and Houtekamer, M.J. and Middelburg, J.J. (2008) A versatile method for stable carbon isotope analysis of carbohydrates by high-performance liquid chromatography/isotope ratio mass spectrometry. Rapid Communications in Mass Spectrometry, 22, 3902-3908. ISSN 0951-4198.

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Official URL: http://dx.doi.org/10.1002/rcm.3804

Abstract

We have developed a method to analyze stable carbon isotope (13C/12C) ratios in a variety of carbohydrates using high-performance liquid chromatography/isotope ratio mass spectrometry (HPLC/IRMS). The chromatography is based on strong anion-exchange columns with low strength NaOH eluents. An eluent concentration of 1 mM resulted in low background signals and good separation of most of the typical plant neutral carbohydrates. We also show that more strongly bound carbohydrates such as acidic carbohydrates can be separated by inclusion of NO as an inorganic pusher ion in the eluent. Analyses of neutral carbohydrate concentrations and their stable carbon isotope ratios are shown for plant materials and marine sediment samples both at natural abundance and for 13C-enriched samples. The main advantage of HPLC/IRMS analysis over traditional gas chromatography based methods is that no derivatization is needed resulting in simple sample treatment and improved accuracy and reproducibility.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:4752
Deposited On:29 Sep 2009 02:00
Last Modified:24 Apr 2012 16:47

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