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Analysis of [U-13C6]glucose in human plasma using liquid chromatography/isotope ratio mass spectrometry compared with two other mass spectrometry techniques

Schierbeek, H. and Moerdijk-Poortvliet, T.C.W. and Akker van den, C.H.P. and Braake te, F.W.J. and Boschker, H.T.S. and Goudoever van, J.B. (2009) Analysis of [U-13C6]glucose in human plasma using liquid chromatography/isotope ratio mass spectrometry compared with two other mass spectrometry techniques. Rapid Communications in Mass Spectrometry, 23, 3824-3830. ISSN 0951-4198.

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Official URL: http://dx.doi.org/10.1002/rcm.4293

Abstract

The use of stable isotope labelled glucose provides insight into glucose metabolism. The 13C-isotopic enrichment of glucose is usually measured by gas chromatography/mass spectrometry (GC/MS) or gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). However, in both techniques the samples must be derivatized prior to analysis, which makes sample preparation more labour-intensive and increases the uncertainty of the measured isotopic composition. A novel method for the determination of isotopic enrichment of glucose in human plasma using liquid chromatography/isotope ratio mass spectrometry (LC/IRMS) has been developed. Using this technique, for which hardly any sample preparation is needed, we showed that both the enrichment and the concentration could be measured with very high precision using only 20 µL of plasma. In addition, a comparison with GC/MS and GC/IRMS showed that the best performance was achieved with the LC/IRMS method making it the method of choice for the measurement of 13C-isotopic enrichment in plasma samples.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:6151
Deposited On:23 Mar 2010 01:00
Last Modified:24 Apr 2012 16:45

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