Joosten, L. and Mulder, P.P.J. and Vrieling, K. and Van Veen, J.A. and Klinkhamer, P.G.L. (2010) The analysis of pyrrolizidine alkaloids in Jacobaea vulgaris; a comparison of extraction and detection methods. Phytochemical Analysis, 21, 197-204. ISSN 0958-0344.
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Official URL: http://dx.doi.org/10.1002/pca.1183
Introduction - Pyrrolizidine alkaloids (PAs) serve an important function in plant defence. Objective - To compare different extraction methods and detection techniques, namely gas chromatography with nitrogen phosphorus detection (GC-NPD) and liquid chromatography tandem mass spectrometry (LC-MS/MS) with quadrupole analysers for analysing PAs in Jacobaea vulgaris. Methodology - Both formic acid and sulfuric acid were tested for PA extraction from dry plant material. For GC-NPD, reduction is required to transform PA N-oxides into tertiary amines. Zinc and sodium metabisulfite were compared as reducing agents. Results - The lowest PA concentration measured with GC-NPD was approximately 0.03 mg/g and with LC-MS/MS 0.002 mg/g. The detection of major PAs by both techniques was comparable but a number of minor PAs were not detected by GC-NPD. With the LC-MS/MS procedure higher concentrations were found in plant extracts, indicating that losses may have occurred during the sample preparation for the GC-NPD method. Zinc proved a more effective reducing agent than sodium metabisulfite. The sample preparation Conclusions - In terms of sensitivity and discrimination, formic acid extraction in combination with LC-MS/MS detection is the method of choice for analysing PAs (both free and N-oxides forms) in plant material.
|Institutes:||Nederlands Instituut voor Ecologie (NIOO)|
|Deposited On:||01 Jul 2010 02:00|
|Last Modified:||04 Sep 2014 10:11|
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