Noonim, P. and Mahakarnchanakul, W. and Nielsen, K.F. and Frisvad, J.C. and Samson, R.A. (2009) Fumonisin B2 production by Aspergillus niger in Thai coffee beans. Food Addit Contam Part A Chem Anal Control Expo Risk Assess, 26, 94-100. ISSN 1944-0057.
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Official URL: http://dx.doi.org/10.1080/02652030802366090
During 2006 and 2007, a total of 64 Thai dried coffee bean samples (Coffea arabica) from two growing sites in Chiangmai Province and 32 Thai dried coffee bean samples (Coffea canephora) from two growing sites in Chumporn Province, Thailand, were collected and assessed for fumonisin contamination by black Aspergilli. No Fusarium species known to produce fumonisin were detected, but black Aspergilli had high incidences on both Arabica and Robusta Thai coffee beans. Liquid chromatography (LC) with high-resolution mass spectrometric (HRMS) detection showed that 67% of Aspergillus niger isolates from coffee beans were capable of producing fumonisins B(2) (FB(2)) and B(4) when grown on Czapek Yeast Agar with 5% NaCl. Small amounts (1-9.7 ng g(-1)) of FB(2) were detected in seven of 12 selected coffee samples after ion-exchange purification and LC-MS/MS detection. Two samples also contained FB(4). This is the first record of freshly isolated A. niger strains producing fumonisins and the first report on the natural occurrence of FB(2) and FB(4) in coffee.
|Institutes:||Centraalbureau voor Schimmelcultures (CBS)|
|Deposited On:||27 Jan 2010 01:00|
|Last Modified:||03 Aug 2010 15:25|
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