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A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex

O'Donnell, K. and Gueidan, C. and Sink, S. and Johnston, P.R. and Crous, P.W. and Glenn, A. and Riley, R. and Zitomer, N. and Colyer, P. and Waalwijk, C. and Lee van der, T.A.J. and Moretti, A. and Kang, S. and Kim, H.K. and Geiser, D.M. and Juba, J. and Baayen, R. P. and Cromey, M. G. and Bithell, S. and Sutton, D.A. and Skovgaard, K. and Ploetz, R. and Kistler, H.C. and Elliott, M. and Davis, M. and Sarver, B.A. (2009) A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genetics and Biology, 46, 936-48. ISSN 1087-1845.

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Official URL: http://dx.doi.org/10.1016/j.fgb.2009.08.006

Abstract

We constructed a two-locus database, comprising partial translation elongation factor (EF-1alpha) gene sequences and nearly full-length sequences of the nuclear ribosomal intergenic spacer region (IGS rDNA) for 850 isolates spanning the phylogenetic breadth of the Fusarium oxysporum species complex (FOSC). Of the 850 isolates typed, 101 EF-1alpha, 203 IGS rDNA, and 256 two-locus sequence types (STs) were differentiated. Analysis of the combined dataset suggests that two-thirds of the STs might be associated with a single host plant. This analysis also revealed that the 26 STs associated with human mycoses were genetically diverse, including several which appear to be nosocomial in origin. A congruence analysis, comparing partial EF-1alpha and IGS rDNA bootstrap consensus, identified a significant number of conflicting relationships dispersed throughout the bipartitions, suggesting that some of the IGS rDNA sequences may be non-orthologous. We also evaluated enniatin, fumonisin and moniliformin mycotoxin production in vitro within a phylogenetic framework.

Item Type:Article
Institutes:Centraalbureau voor Schimmelcultures (CBS)
ID Code:6900
Deposited On:27 Jan 2010 01:00
Last Modified:03 Aug 2010 15:25

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