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Highly Efficient ENU Mutagenesis in Zebrafish.

Bruijn de, E. and Cuppen, E. and Feitsma, H. (2009) Highly Efficient ENU Mutagenesis in Zebrafish. Methods in Molecular Biology, 546, 3-12. ISSN 1064-3745.

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Official URL: http://depot.knaw.nl/6945

Abstract

ENU (N-ethyl-N-nitrosourea) mutagenesis is a widely accepted and proven method to introduce random point mutations in the genome. Because there are no targeted knockout strategies available for zebrafish so far, random mutagenesis is currently the preferred method in both forward and reverse genetic approaches. To obtain high-density mutagenized zebrafish, six consecutive ENU treatments are applied at weekly intervals to adult male zebrafish by bathing them in ENU solution. With this procedure an average germ line mutation load of one mutation every 1.0 x 10(5)-1.5 x 10(5) basepairs is reached routinely in our lab.

Item Type:Article
Institutes:Hubrecht Instituut
ID Code:6945
Deposited On:15 Dec 2009 01:00
Last Modified:10 Aug 2011 14:57

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