Claycomb, J.M. and Batista, P.J. and Pang, K.M. and Gu, W. and Vasale, J.J. and Wolfswinkel van, J.C. and Chaves, D.A. and Shirayama, M. and Mitani, S. and Ketting, R.F. and nte D Co, J.r. and Mello, C.C. (2009) The Argonaute CSR-1 and its 22G-RNA cofactors are required for holocentric chromosome segregation. Cell, 139, 123-34. ISSN 0092-8674.
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RNAi-related pathways regulate diverse processes, from developmental timing to transposon silencing. Here, we show that in C. elegans the Argonaute CSR-1, the RNA-dependent RNA polymerase EGO-1, the Dicer-related helicase DRH-3, and the Tudor-domain protein EKL-1 localize to chromosomes and are required for proper chromosome segregation. In the absence of these factors chromosomes fail to align at the metaphase plate and kinetochores do not orient to opposing spindle poles. Surprisingly, the CSR-1-interacting small RNAs (22G-RNAs) are antisense to thousands of germline-expressed protein-coding genes. Nematodes assemble holocentric chromosomes in which continuous kinetochores must span the expressed domains of the genome. We show that CSR-1 interacts with chromatin at target loci but does not downregulate target mRNA or protein levels. Instead, our findings support a model in which CSR-1 complexes target protein-coding domains to promote their proper organization within the holocentric chromosomes of C. elegans.
|Deposited On:||21 Dec 2009 01:00|
|Last Modified:||13 Oct 2010 12:51|
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