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Tissue- and expression level-specific chromatin looping at maize b1 epialleles.

Louwers, M. and Bader, R. and Haring, M. and Driel van, R. and Laat de, W. and Stam, M. (2009) Tissue- and expression level-specific chromatin looping at maize b1 epialleles. Plant Cell, 21, 832-42. ISSN 1040-4651.

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Abstract

This work examines the involvement of chromatin looping in the transcriptional regulation of two epialleles of the maize (Zea mays) b1 gene, B-I and B'. These two epialleles are tissue-specifically regulated and are involved in paramutation. B-I and B' are expressed at high and low levels, respectively. A hepta-repeat approximately 100 kb upstream of the transcription start site (TSS) is required for both paramutation and high b1 expression. Using chromosome conformation capture, we show that the hepta-repeat physically interacts with the TSS region in a tissue- and expression level-specific manner. Multiple repeats are required to stabilize this interaction. High b1 expression is mediated by a multiloop structure; besides the hepta-repeat, other sequence regions physically interact with the TSS as well, and these interactions are epiallele- and expression level-specific. Formaldehyde-assisted isolation of regulatory elements uncovered multiple interacting regions as potentially regulatory.

Item Type:Article
Institutes:Hubrecht Instituut
ID Code:6956
Deposited On:24 Dec 2009 01:00
Last Modified:13 Oct 2010 12:51

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