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Unbalancing the phosphatidylinositol-4,5-bisphosphate-cofilin interaction impairs cell steering.

Leyman, S. and Sidani, M. and Ritsma, L. and Waterschoot, D. and Eddy, R. and Dewitte, D. and Debeir, O. and Decaestecker, C. and Vandekerckhove, J. and Rheenen van, J. and Ampe, C. and Condeelis, J. and Troys Van, M. (2009) Unbalancing the phosphatidylinositol-4,5-bisphosphate-cofilin interaction impairs cell steering. Molecular Biology of the Cell, 20, 4509-23. ISSN 1059-1524.

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Cofilin is a key player in actin dynamics during cell migration. Its activity is regulated by (de)phosphorylation, pH, and binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P(2)]. Here, we here use a human cofilin-1 (D122K) mutant with increased binding affinity for PI(4,5)P(2) and slower release from the plasma membrane to study the role of the PI(4,5)P(2)-cofilin interaction in migrating cells. In fibroblasts in a background of endogenous cofilin, D122K cofilin expression negatively affects cell turning frequency. In carcinoma cells with down-regulated endogenous cofilin, D122K cofilin neither rescues the drastic morphological defects nor restores the effects in cell turning capacity, unlike what has been reported for wild-type cofilin. In cofilin knockdown cells, D122K cofilin expression promotes outgrowth of an existing lamellipod in response to epidermal growth factor (EGF) but does not result in initiation of new lamellipodia. This indicates that, next to phospho- and pH regulation, the normal release kinetics of cofilin from PI(4,5)P(2) is crucial as a local activation switch for lamellipodia initiation and as a signal for migrating cells to change direction in response to external stimuli. Our results demonstrate that the PI(4,5)P(2) regulatory mechanism, that is governed by EGF-dependent phospholipase C activation, is a determinant for the spatial and temporal control of cofilin activation required for lamellipodia initiation.

Item Type:Article
Institutes:Hubrecht Instituut
ID Code:6960
Deposited On:24 Dec 2009 01:00
Last Modified:13 Oct 2010 12:51

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