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Isolation of deletion alleles by G4 DNA-induced mutagenesis.

Pontier, D.B. and Kruisselbrink, E. and Guryev, V. and Tijsterman, M. (2009) Isolation of deletion alleles by G4 DNA-induced mutagenesis. Nature Methods, 6, 655-7. ISSN 1548-7091.

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Abstract

Metazoan genomes contain thousands of sequence tracts that match the guanine-quadruplex (G4) DNA signature G(3)N(x)G(3)N(x)G(3)N(x)G(3), a motif that is intrinsically mutagenic, probably because it can form secondary structures during DNA replication. Here we show how and to what extent this feature can be used to generate deletion alleles of many Caenorhabditis elegans genes.

Item Type:Article
Institutes:Hubrecht Instituut
ID Code:6965
Deposited On:04 Jan 2010 01:00
Last Modified:13 Oct 2010 12:51

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