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(Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage

Balk, M. and Mehboob, F. and Gelder Van, T. and Rijpstra, W.I.C. and Sinninghe Damsté, J.S. and Stams, A.J.M. (2010) (Per)chlorate reduction by an acetogenic bacterium, Sporomusa sp., isolated from an underground gas storage. Applied Microbiology and Biotechnology, 88, 595-603. ISSN 0175-7598.

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Official URL: http://dx.doi.org/10.1007/s00253-010-2788-8

Abstract

A mesophilic bacterium, strain An4, was isolated from an underground gas storage reservoir with methanol as substrate and perchlorate as electron acceptor. Cells were Gram-negative, spore-forming, straight to curved rods, 0.5–0.8 μm in diameter, and 2–8 μm in length, growing as single cells or in pairs. The cells grew optimally at 37°C, and the pH optimum was around 7. Strain An4 converted various alcohols, organic acids, fructose, acetoin, and H2/CO2 to acetate, usually as the only product. Succinate was decarboxylated to propionate. The isolate was able to respire with (per)chlorate, nitrate, and CO2. The G+C content of the DNA was 42.6 mol%. Based on the 16S rRNA gene sequence analysis, strain An4 was most closely related to Sporomusa ovata (98% similarity). The bacterium reduced perchlorate and chlorate completely to chloride. Key enzymes, perchlorate reductase and chlorite dismutase, were detected in cell-free extracts.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:7760
Deposited On:07 Oct 2010 02:00
Last Modified:06 Sep 2011 15:18

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