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Distribution and diversity of gallionella-like neutrophilic iron oxidizers in a tidal freshwater marsh

Wang, J. and Vollrath, S. and Behrends, T. and Bodelier, P.L.E. and Muyzer, G. and Den Oudsten, F. and Meima-Franke, M. and Cappellen, P. and Laanbroek, H.J. (2011) Distribution and diversity of gallionella-like neutrophilic iron oxidizers in a tidal freshwater marsh. Applied and Environmental Microbiology, 77, 2337-2344. ISSN 0099-2240.

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Official URL: http://dx.doi.org/10.1128/AEM.02448-10

Abstract

Microbial iron oxidation is an integral part of the iron redox cycle in wetlands. Nonetheless, relatively little is known about the composition and ecology of iron-oxidizing communities in the soils and sediments of wetlands. In this study, sediment cores were collected across a freshwater tidal marsh in order to characterize the iron-oxidizing bacteria (FeOB) and to link their distributions to the geochemical properties of the sediments. We applied recently designed 16S rRNA primers targeting Gallionella-related FeOB by using a nested PCR-denaturing gradient gel electrophoresis (DGGE) approach combined with a novel quantitative PCR (qPCR) assay. Gallionella-related FeOB were detected in most of the samples. The diversity and abundance of the putative FeOB were generally higher in the upper 5 to 12 cm of sediment than in deeper sediment and higher in samples collected in April than in those collected in July and October. Oxygen supply by macrofauna appears to be a major force in controlling the spatial and temporal variations in FeOB communities. The higher abundance of Gallionella-related FeOB in April coincided with elevated concentrations of extractable Fe(III) in the sediments. Despite this coincidence, the distributions of FeOB did not exhibit a simple relationship to the redox zonation inferred from the geochemical depth profiles.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:8359
Deposited On:17 Dec 2010 01:00
Last Modified:24 Apr 2012 16:43

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