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Production of indole-3-acetic acid by the cyanobacterium Arthrospira platensis strain MMG-9

Ahmed, M. and Stal, L.J. and Hasnain, S. (2010) Production of indole-3-acetic acid by the cyanobacterium Arthrospira platensis strain MMG-9. Journal of Microbiology and Biotechnology, 20, 1259-1265. ISSN 1017-7825.

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Official URL: http://dx.doi.org/10.4014/jmb.1004.04033

Abstract

The filamentous cyanobacterium Arthrospira platensis strain MMG-9 was isolated from a rice field. The ability of this strain to synthesize the bioactive compound indole- 3-acetic acid (IAA) was demonstrated. IAA was extracted from the culture of A. platensis strain MMG-9 and its identity was confirmed by thin-layer chromatography (TLC) as well as by high- erformance liquid chromatography (HPLC). The IAA precursor L-tryptophan was required for IAA biosynthesis. Released IAA increased with the increase of the initial concentration of L-tryptophan in the medium and with the incubation time. A. platensis strain MMG-9 accumulated more IAA than it released into the medium. The bioactivity of the secreted IAA was shown by its effect on the formation of roots by Pisum sativum. There was a significant positive effect of the supernatant of cultures of A. platensis strain MMG-9 on the number of lateral roots of P. sativum, whereas a negative effect on root length was observed.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:9059
Deposited On:04 Jan 2011 01:00
Last Modified:24 Apr 2012 16:41

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