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Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

Mela, F. and Fritsche, K. and De Boer, W. and Van Veen, J.A. and Graaff de, L. and Van den Berg, M. and Leveau, J.H.J. (2011) Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger. ISME Journal, 5, 1494-1504. ISSN 1751-7362.

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Official URL: http://dx.doi.org/10.1038/ismej.2011.29

Abstract

Interactions between bacteria and fungi cover a wide range of incentives, mechanisms and outcomes. The genus Collimonas consists of soil bacteria that are known for their antifungal activity and ability to grow at the expense of living fungi. In non-contact confrontation assays with the fungus Aspergillus niger, Collimonas fungivorans showed accumulation of biomass concomitant with inhibition of hyphal spread. Through microarray analysis of bacterial and fungal mRNA from the confrontation arena, we gained new insights into the mechanisms underlying the fungistatic effect and mycophagous phenotype of collimonads. Collimonas responded to the fungus by activating genes for the utilization of fungal-derived compounds and for production of a putative antifungal compound. In A. niger, differentially expressed genes included those involved in lipid and cell wall metabolism and cell defense, which correlated well with the hyphal deformations that were observed microscopically. Transcriptional profiles revealed distress in both partners: downregulation of ribosomal proteins and upregulation of mobile genetic elements in the bacteria and expression of endoplasmic reticulum stress and conidia-related genes in the fungus. Both partners experienced nitrogen shortage in each other’s presence. Overall, our results indicate that the Collimonas/ Aspergillus interaction is a complex interplay between trophism, antibiosis and competition for nutrients.

Item Type:Article
Institutes:Nederlands Instituut voor Ecologie (NIOO)
ID Code:9166
Deposited On:02 Sep 2011 02:00
Last Modified:24 Apr 2012 16:41

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