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ENU mutagenesis to generate genetically modified rat models

Boxtel van, R. and Gould, M. and Cuppen, E. and Smits, B.M. (2010) ENU mutagenesis to generate genetically modified rat models. Methods in Molecular Biology, 597, 151-67. ISSN 1064-3745.

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Official URL: http://dx.doi.org/10.1007/978-1-60327-389-3_11

Abstract

The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.

Item Type:Article
Institutes:Hubrecht Instituut
ID Code:9445
Deposited On:29 Jan 2010 01:00
Last Modified:07 Sep 2011 17:01

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