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In situ hybridization to identify gut stem cells

Gregorieff, A. and Clevers, H. (2010) In situ hybridization to identify gut stem cells. Current Protocols in Stem Cell Biology, Chapter 2, Unit-2F 1. ISSN 1941-7322.

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In recent years, considerable effort has been directed towards identifying the repertoire of genes specifically expressed in adult stem cells. In this unit, we describe an in situ hybridization protocol adapted for the analysis of gene expression in the intestinal mucosa. This methodology allows researchers to quickly visualize the expression profile of putative stem cell markers with a high degree of sensitivity and resolution.

Item Type:Article
Institutes:Hubrecht Instituut
ID Code:9457
Deposited On:04 Jan 2011 01:00
Last Modified:07 Sep 2011 17:01

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